Abstract:

BACKGROUND: Bone marrow stromal stem cells can be widely obtained, easily separated, rapidly amplified, and with the potential of multiple directional differentiation, and are suitable for the characteristics of autotransplantation. Simultaneously, bone marrow stromal stem cells are suitable seed cells in bone tissue engineering.
OBJECTIVE: To summarize and analyze the advantage of transgene or its complex stent for repair of bone defect using bone marrow stromal stem cells as seed cells.
METHODS: Biomedical Bibliographic Database in Western Journals and China National Knowledge Infrastructure were searched for articles concerning isolation, culture and application of bone marrow stromal stem cells to bone defect as well as bone tissue engineering published from 1990 to 2009. The key words were “bone marrow stem cells, repairing, bone defect”. Repetitive articles were excluded. Finally, 28 articles were further summarized.
RESULTS AND CONCLUSION: This article summarized the preponderance of bone marrow stromal stem cells as seed cells, isolation and culture of bone marrow stromal stem cells, osteogenic induction of bone marrow stromal stem cells and repair of bone defect using bone marrow stromal stem cells. The methods bring light prospects for the treatment of bone defect, such as bone marrow stromal stem cells as seed cells combined with suitable stent material, or bone marrow stromal stem cells as target cells to introduce endogenous target gene to induce osteogenic formation. However, some problems require further investigations, including purification of bone marrow stromal stem cells, proliferation and suitable condition for differentiation of bone marrow stromal stem cells, which kind of factors can effectively promote new bone formation, carrier material and in vivo implantation manner, as well as how to combine bone tissue engineering and gene therapy.